FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21006151
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA52750.09.0073319
CAACACT71600.07.44038538
AGTGAGT37050.06.87876461
GTGAGTA39750.06.339112
TTAGACC20402.450017E-55.9992297
GTTAGAC20502.6407602E-55.96996456
ACATAAA126900.05.9676361
TAGTTAG20652.9516379E-55.92674064
TAACCCC47600.05.74713339
GAGCACA205100.05.47562369
ACCAATC78350.05.42112876
TCGGAAG237250.05.37111473
TACCAAT73200.05.3107935
GATCGGA211800.05.29450461
CATAAAT134700.05.29133132
AGAGCAC217300.05.1019688
Produced by FastQC (version 0.11.5)