FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15375957
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTA35750.016.51298
CTACACT40050.014.5602247
ACACTAT48350.011.9118449
CTAGCTC10650.010.68023735-39
ACTACAC69950.08.748166
ACAACTA82050.08.1660233
TCTAGCT14800.08.07457635-39
CTCTAGC15800.07.74578135-39
TCTCTAG15450.07.641678335-39
ACGTTAA32200.07.38353444
TACAACT90950.07.05004932
ATACAAC100100.06.78333331
AACTACA99950.06.0503715
TACGTTA40557.2759576E-125.8631273
GTTACGT47851.8189894E-115.34048131
Produced by FastQC (version 0.11.5)