FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15375957
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTA33750.015.9978378
CTACACT40800.012.350877
ACACTAT45500.011.3918689
CTAGCTC12950.08.00506935-39
TCTAGCT15100.07.342030535-39
ACTACAC73650.07.135266
TCTCTAG15600.07.10670935-39
CTCTAGC16100.07.06486135-39
ACAACTA87950.06.96102243
ACGTTAA29201.1277734E-106.6599484
AACTACA96300.06.27936175
TACAACT95300.06.27581552
TACGTTA38557.2759576E-115.79198273
ATACAAC101050.05.51833061
CTAGATC9409.276846E-115.2076983140-144
ACTATAG27900.05.00577510-14
Produced by FastQC (version 0.11.5)