FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17062036
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGG77250.013.3272336
TTTACTA77900.012.5715232
ACTATGG78100.012.5369045
TACTATG86300.011.5135764
TTACTAT97550.010.555593
ATTTACT96850.010.2073591
ATTTGAG105650.09.9763721
TATGGGC106850.09.837417
ATGGGCC109550.09.4635458
TTGAGGG134350.08.8434143
TGAGGGC165800.06.77457334
GCAAGAC174000.06.5374989
TTTGAGG183550.06.2769992
TTAGTAG39050.05.862726765-69
TGGGCCG189500.05.6988319
CTTAGAG40950.05.344792130-134
TGCTTAG43850.05.2867837125-129
AGGGCAA238800.05.1554226
GCTTAGA42650.05.0979905130-134
Produced by FastQC (version 0.11.5)