FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14252333
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCAGCAACACTATCTTTAACTTTCTCACCTTCAGTTACACATTAAAT143190.10046776201482241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACAC104750.022.542687
TAGACCC33650.022.0362638
TTAGACC35550.020.6560067
CAACACT115400.020.5870388
GTTAGAC37950.019.53946
GTTTCAG156250.015.7947061
AACACTA163800.014.8115949
AGACCCC53600.014.5059019
CAGCAAC178100.013.7840315
TAGTTAG69450.011.1953394
TTAGCCC42700.011.094488120-124
AGCAACA236000.010.7988396
TAGACTC42900.010.706751135-139
AGTTAGA74700.010.4085195
TCAGCAA248200.010.0939984
TTTCAGC293750.08.4336522
ATTAGCC59900.07.980875120-124
ATGTAGT120250.07.74919751
ACACTAG65300.07.673309370-74
TTCAGCA334250.07.58153253
Produced by FastQC (version 0.11.5)