FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11897133
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA46300.025.966747
ACACTAT48100.024.9950128
CACTATA56400.021.4443139
AAACACT83550.015.165016
ACCAAAC88900.014.0093223
CAAACAC90950.013.7728245
TAGCCCT51600.012.9807442
GCCCTTA54300.012.7275324
CCAAACA98450.012.6503694
TATTTAG64800.010.5543269
TACCAAA140700.09.469872
TACACTA34650.08.7261476
GTAGCCC76200.08.7113991
CACCCTA28100.08.55691775-79
CCCTTAT84350.08.5347615
ATACCAA169350.08.2271541
AGCCCTT94250.07.79097133
TTAGGCA42750.07.71346760-64
CCTTATT101000.07.62673856
TTTAGGC43450.07.58919960-64
Produced by FastQC (version 0.11.5)