FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15141349
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT240200.15863844100020413Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA146800.025.06491
TCGGAAG195800.019.0465563
GAGCGTC213950.017.6662759
AGAGCGT216550.017.420928
ATCGGAA225850.016.7354982
AAGAGCG269150.014.3105857
CGGAAGA292100.013.06302454
GAAGAGC346750.011.39876
GGAAGAG353350.011.1042555
TAGATCT73850.010.5478740-44
CAGTGTA81900.09.61659535-39
AGTGTAG88000.08.54094240-44
TCAGTGT98250.08.26541235-39
GTAGATC100800.07.98489540-44
GTAGGGA112300.07.41098715-19
AGTGTTC108600.07.31879425-29
AGATCTC107100.07.28806845-49
TGTAGGG114750.07.24020915-19
GAGTGTT114350.07.21520625-29
TAGGGAA117400.07.17489815-19
Produced by FastQC (version 0.11.5)