FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19005304
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT51000.026.2647421
TGAGTAG52400.025.2814043
GTGAGTA56450.024.2666782
TAGCATG64500.022.9931968
GAGTAGG64600.020.3943754
TAGGAGA110550.012.1128417
GTAGGAG129100.010.7069756
CCTTTAG146750.010.645684
AGGAGAG128950.010.6077698
AGTAGGA156050.09.3192175
GGAGAGG158350.09.13839159
TTAGCAT186700.08.753337
CTAGGAG32950.08.65229650-54
CTTTAGC214000.07.90580135
AGCATGA220750.07.3705449
TTTAGCA242400.07.0686456
GTTCCTT261050.06.83231541
TCCTTTA300400.06.18350123
TTCTAGG54850.05.53894950-54
CTAACTA56600.05.520302125-129
Produced by FastQC (version 0.11.5)