FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19005304
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT51450.024.1525461
TGAGTAG52300.023.5358283
TAGCATG69350.023.1468098
GTGAGTA58450.021.3080942
GAGTAGG62600.019.7783094
TAGGAGA111450.011.3675397
CCTTTAG148650.011.3314634
GTAGGAG128350.010.4312446
AGGAGAG136050.09.4179028
TTAGCAT182850.09.330127
AGTAGGA155300.08.8528065
CTTTAGC213550.08.1570825
AGCATGA216450.08.114569
GGAGAGG162050.08.0401159
CTAGGAG34550.07.91900250-54
TTTAGCA241900.07.3796346
GTTCCTT264500.07.10242751
TCCTTTA303750.06.0904853
ACCCTAT70750.05.454563685-89
TTCTAGG54450.05.39507250-54
Produced by FastQC (version 0.11.5)