FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16458309
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTTCAGGTGTAGATCT965340.5865365633857038Illumina Single End PCR Primer 1 (97% over 36bp)
AAAGGTACACAAGACCCCTACTTTTAACATTTCCCAACAGGTTTTTAACA220120.13374399520631192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA267000.054.4362681
GAGCGTC314500.046.413379
AGAGCGT316150.046.307598
TCGGAAG323500.045.301953
GTACACA94100.043.3148085
ATCGGAA346050.042.4539452
AAGAGCG365450.040.3263177
ACACAAG112600.035.869327
GGTACAC114000.035.691954
CGGAAGA413450.035.5157474
TACACAA122450.033.5186
GGAAGAG453050.032.584975
GAAGAGC457700.032.4234436
AGGTACA152800.026.5816843
CACAAGA136350.026.1830948
AAGGTAC162250.024.8559322
TAGATCT154650.019.52249140-44
GTAGATC173500.017.55912840-44
ACAAGAC213950.017.056569
AGATCTC176450.016.85391845-49
Produced by FastQC (version 0.11.5)