FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14801775
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGT71050.015.7829361
GGTGTTT123100.011.8129451
GTGTGTC63900.011.7193612
GGTGTTC152250.011.1193321
GGTATTT208300.010.975341
GGTATAC65050.010.8993091
GGTATAA138300.010.7238771
GGTGTTG149800.010.72164251
GGTGTTA64800.010.7180651
GGTGTGA85100.010.5417391
GGTATAT172600.09.8921651
GGTGTAA89850.09.6623621
GGTGTAG115700.09.4419951
GGTGTAT114600.09.2801051
GGTGTGG123750.09.2370231
GGTATTA111450.09.0230821
GGTATTG233000.08.8493031
GGTGAAA240700.08.806671
GGTGTAC94800.08.7762631
GGTATCA177600.08.6360081
Produced by FastQC (version 0.11.5)