FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16858878
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATCTCGTAT169430.10049897745271066TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA185350.015.5380249
AGAGCAC213050.013.6530048
GATCGGA257200.011.5327541
TCGGAAG261300.011.2982583
AAGAGCA273500.010.8459677
ATCGGAA301900.010.1842232
GGAAGAG331050.09.1779825
GAAGAGC373150.08.2196726
CGGAAGA374900.08.009414
AGTCACG87100.06.76161425-29
CAGTCAC90500.06.587141525-29
ACGTACG96150.06.319877630-34
CCAGTCA105350.05.808976725-29
CTCGTAT107100.05.606656640-44
TCACGTA105350.05.56295330-34
CACGTAC108950.05.445220530-34
TCTCGTA118450.05.36118640-44
TACGCAA120050.05.26588135-39
CAAATCT125550.05.023727435-39
Produced by FastQC (version 0.11.5)