FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283528
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG6553.310888E-713.1904763
CTAGCCG7702.8621991E-510.2852376
CCCTAAG13052.0576408E-89.3788756
TCTAGCC7100.00160777959.1263375
ATCTAGC9653.428625E-58.9529524
CTAAGAG15703.3711767E-67.33723938
ACCCTAA21451.2089222E-66.3773265
TACCCTA22604.3815817E-76.3713864
CCTAAGA19906.8251335E-45.42688377
Produced by FastQC (version 0.11.5)