FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13129501
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTAT137660.1048478536998474TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT33650.031.8376161
TAAGGGG33150.029.3200179
GTGAGTA39450.027.1937922
GATCGGA120200.018.9098911
GAGCACA121800.018.2652329
ATAAGGG60900.016.3145778
GAGTATA76300.014.4372954
TCGGAAG165150.014.3868553
AGAGCAC163250.013.8481088
TGAGTAT81450.013.7899923
ATCGGAA182750.013.0801042
CTAGGAT35300.012.8084730-34
TATAAGG82000.012.1165567
GTATAAG80800.012.029196
CACTAGG37750.011.71018430-34
AGTCACT42550.010.72758725-29
GTCACTA42300.010.65482425-29
ACTAGGA46550.09.7748430-34
CAGTCAC51400.09.27272125-29
AAGAGCA259200.09.2218287
Produced by FastQC (version 0.11.5)