FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15712462
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA48100.014.9670437
CACTATA52800.014.0437939
ACACTAT52800.013.6347498
AAACACT88600.08.7754856
ACCAAAC94950.08.1193473
CCAAACA112300.06.92560634
TACCAAA130050.06.37113762
CCTAATG32902.8012437E-106.1518361
CCCCCTA31600.06.0610027125-129
CAAACAC134000.06.01721765
TAAATAT210650.05.46813967
ATACCAA160100.05.372771
GGGGGGT35200.05.3585315115-119
AATAAAT204350.05.3549015
AATATAA211300.05.24689449
CAAAATA202450.05.16035652
ATAAATA224750.05.157126
CTAATGA46603.8744474E-105.10220052
ACAAAAT208900.05.08652831
GTACGTA41450.05.07186195-99
Produced by FastQC (version 0.11.5)