FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15712462
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA52500.015.3507127
ACACTAT52350.014.84526358
CACTATA53150.014.6219119
AAACACT92250.09.2041876
ACCAAAC92700.08.7739053
CCAAACA108650.07.75081874
TACCAAA127200.07.2427922
CAAACAC130550.06.94485955
CCCCCTA30650.06.8590307125-129
CAAAATA195900.06.65008642
CCTAATG38900.06.55515771
AATAAAT209950.06.5118975
GTACGTA41950.06.45335695-99
ATACCAA155000.06.39251761
ACAAAAT210450.06.3699141
TAAATAT213550.06.36842447
AATATAA218000.06.2716349
ATAAATA226600.06.1921936
TAGGCTA45050.06.04259285-89
AAATATA249400.05.77051268
Produced by FastQC (version 0.11.5)