FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20497314
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCCC49200.035.2950743
CCCCCCA59950.029.0732294
TACCCCC72650.024.7952022
CCACAGT76950.023.2938148
CACAGTA77250.023.1101679
CCCCACA102250.017.882616
CCCCCAC104100.017.150025
CCTAGGT21800.015.85109285-89
ATACCCC130100.014.571111
CCCACAG127700.014.3747137
TCCTAGG31250.011.6577480-84
CTAGGTA35400.010.33082385-89
TTCCTAG43300.08.64628180-84
ATGCAAT135750.08.2196915
CTCACTA45800.08.11056840-44
GATGCAA151550.07.03352264
GTCTCAC59350.06.622930535-39
TGATGCA193600.05.7316223
TCTCACT69700.05.721949640-44
TGCAATA190500.05.70614056
Produced by FastQC (version 0.11.5)