FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17521705
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG34100.011.3993666
GTAGCAT38650.010.0574549
CTGTAGC50100.08.0462157
TGTAGCA57800.07.47258668
GATCTAA13650.06.855598445-49
TAGTTAG15900.05.613941730-34
TAGTCAT44251.6370905E-115.53104266
CGTTAGT17000.05.41991570-74
ACGTTAG17150.05.28856570-74
TCCCTAA21100.00148073755.12103372
Produced by FastQC (version 0.11.5)