FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14658729
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA40900.014.082254
GTGCACA56450.011.477417
TGTGCAC59550.011.2425956
CCCTTAG52650.011.075215
TAGAGCC56400.010.72174559
TCGTGTG58500.010.7070593
GCACACA65400.010.1268759
CTTAGAG65750.09.74457
TTAGAGC62900.09.4993268
TGCACAC71850.09.1175888
CCTTAGA69900.08.8569996
TTCGTGT73350.08.8338342
GTGTGCA73450.08.6249435
CGTGTGC76200.08.1254764
TGCCCCT76800.07.9682792
GCCCCTT78650.07.78084953
GTTCGTG98450.07.24251461
CTGCCCC117500.05.6929441
Produced by FastQC (version 0.11.5)