FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18585752
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA64000.014.9651997
ACACTAT64650.014.7012518
CACTATA66450.014.1937129
AAACACT106600.09.8629436
CAAACAC108200.09.3843185
ACCAAAC119350.08.4472943
CCAAACA129500.07.84081754
GCCCTTA70700.05.8058724
TAGCCCT81550.05.47666932
TACCAAA206250.05.41360472
TATTTAG87100.05.04233079
Produced by FastQC (version 0.11.5)