FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17537222
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA67650.016.1694379
GCCACTC82950.013.27376658
AGCCACT116400.09.8918197
CCGTTAA70300.09.317874
AAGCCAC122950.09.2475756
CGTTAAA82250.08.3116215
CCAGCTA58200.07.79198654
TCCGTTA102300.07.317913
TACAAAG188700.07.0574292
GGCCTAA33650.06.84284575-79
ACAAAGC188000.06.62395863
GTTAAAT112200.06.6060776
CTCACTA44550.05.588980715-19
GCTACTA85150.05.57787137
ATACAAA234200.05.55995561
AAAGCCA231250.05.22787955
CTACTAA88950.05.01608478
Produced by FastQC (version 0.11.5)