FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16296072
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC141950.011.8155088
GAGCACA145450.011.5311889
GATCGGA167800.010.1921881
TCGGAAG215850.08.1745883
ATCGGAA239050.07.7427672
AAGAGCA240200.07.49190437
GAAGAGC290250.06.4232196
GGAAGAG298050.06.30342535
CGGAAGA329700.05.63266564
AGTCACA65950.05.501210725-29
ACACGTA63800.05.43873230-34
Produced by FastQC (version 0.11.5)