FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12862706
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA32850.026.5166449
GCCACTC61200.014.3508298
AGCCACT68950.012.1113437
CGTTAAA52750.010.6448785
AAGCCAC80900.010.4113236
CCGTTAA45450.09.02894
CCTAGAC6400.08.998676120-124
TACAAAG112350.08.0741432
CTCACTA22700.07.991775515-19
GTTAAAT54300.07.954636
ACAAAGC112700.07.79354243
ACCACTA46300.07.46326458
CCCTAAG13703.2932243E-57.35658176
GAGCACA77300.07.26413159
ATACAAA132650.06.647121
CACCACT53350.06.61196047
ATCCTAG10450.06.475652115-119
AAAGCCA148400.06.35486035
ACGTTAA24001.9376603E-76.299444
TCCGTTA75600.06.09476763
Produced by FastQC (version 0.11.5)