FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14209933
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTA36500.023.8645720-24
CCTAGCT36500.023.82512520-24
CTAACTA44100.019.91734925-29
GGTCCTA47200.018.3634735-39
AGCTAAC48200.018.10158720-24
GCTAACT49150.017.958825-29
TAGCTAA51000.017.3618520-24
CCTAAGG50000.017.2485740-44
GTCCTAA51200.017.12567135-39
TCCTAAG52550.016.54872335-39
CCCTAGC15000.016.41330120-24
TCCTAGC14900.016.13694420-24
CTAAGGT55100.016.04401640-44
ACCTAGC13300.015.91317320-24
GCCTAGC13950.013.52035820-24
TAGCGAA72950.012.29575245-49
TAAGGTA75900.011.950747540-44
GTAGCGA77050.011.80964245-49
TAACTAT77600.011.63445725-29
CCAGTGA83850.010.97263450-54
Produced by FastQC (version 0.11.5)