FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14415439
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT48950.034.5658841
GTGAGTA58550.028.406492
TAACCCC60450.027.1563269
CTACACT67700.019.6750377
TACACTA67450.019.4277238
ACGTTAA39250.018.527394
ACTACAC76700.016.9908736
ACACTAT77650.016.5048319
ATAACCC118400.014.4121628
TACGTTA51700.013.7872343
GTTACGT56100.013.3475941
GTATAAC129150.013.2682926
TGAGTAT128500.013.1112843
GAGTATA134250.012.8178784
ACAACTA111800.012.3005373
TATAACC152850.011.7291477
TACAACT119800.011.2387312
CTAGCTC39250.011.11643315-19
CGTTAAA64250.010.8700395
ATACAAC123500.010.6105271
Produced by FastQC (version 0.11.5)