FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005184991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005184991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15907458
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT195430.12285432405353514Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA185200.017.2782821
AGAGCGT214850.014.5602568
TCGGAAG231200.013.7319593
ATCGGAA260650.012.4974142
AAGAGCG256450.012.3673967
GAGCGTC259500.012.2216549
GAAGAGC299000.011.0654696
GGAAGAG305100.010.8435145
TAGATCT70300.09.25673140-44
CGGAAGA364000.09.1014434
AGTGTAG76350.08.48551240-44
GAGTGTT93500.07.11379125-29
CAGTGTA94400.06.9384735-39
GTAGATC99150.06.67943440-44
AGAGTGT100150.06.67018325-29
TCAGTGT103850.06.55660435-39
GTAGGGA100300.06.47400215-19
AGATCTC108700.06.317553545-49
AGTGTTC107350.06.289867425-29
GTGTAGA113400.06.23365840-44
Produced by FastQC (version 0.11.5)