FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999029
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA32100.016.8198976
CTTAGTC23050.013.4296478
CCCTACT41650.012.9632348
CCTTAGT30250.011.4230737
TTAGTCC27750.011.1563429
TCCCTAC52100.010.3631237
CCTACTT62350.09.0068829
TCCTTAG38700.08.7428696
GAGCCCA58950.05.6181129
TCTCCCT99950.05.18588165
Produced by FastQC (version 0.11.5)