FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14635256
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC108200.012.0440469
ATGGCTC110300.011.88017755
GGCTCAA106600.011.5495757
TGGCTCA122400.010.6469256
TCTGATG135750.09.55013751
GCTCAAA146700.09.2267848
GATGGCT158000.08.4302744
CCTCTAG13050.06.510143350-54
CTGATGG228200.05.8685632
TGATGGC256850.05.8585993
CTCTAGG12150.05.807227650-54
CTCGGGT47950.05.58574820-24
Produced by FastQC (version 0.11.5)