FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14421865
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA37000.021.9875286
CCCTACT40400.020.4935048
TCCCTAC52550.016.1662397
CCTACTT62150.013.3217859
GTATGCT95950.010.4039861
TTAGTCC21550.010.356679
CTTAGTC23850.09.6596488
CCTTAGT26700.08.3589197
GCTCCCT99900.08.2155955
TATGCTC107750.08.08536152
TGCTCCC122350.07.0611754
TCCTTAG37850.06.6573576
TACTAAC30650.05.872227790-94
ATGCTCC153100.05.83108233
CTAACAT36100.05.504213390-94
ACTAACA34250.05.46520190-94
TAACATG38250.05.383044790-94
Produced by FastQC (version 0.11.5)