FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14783175
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAACACCACTACTCTATAGCTATCTAACACATATGTCTCATAGCCTT168820.1141973899382237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA90150.027.3906158
ACAACAC102250.024.593433
ACACCAC101450.024.4816366
CACCACT102650.024.4759677
ACGTTAA79050.021.862824
GTTACGT78500.021.8270511
CCACTAC113000.021.7881859
CGTTAAA84350.021.16625
TACGTTA97300.018.3652063
CAACACC141050.018.0728654
AACACCA159600.016.3738235
ATACAAC166200.015.6163611
TACAACA173150.014.897592
GTTAAAT137850.013.6303556
TTACGTT149650.012.0851622
CCTAGAC43450.011.466918120-124
AGGCCCC47000.011.24446875-79
GGCCCCC48050.010.93881275-79
TAGCCCC53200.010.01391285-89
CTAGACC56900.09.060061120-124
Produced by FastQC (version 0.11.5)