FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849953
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA52300.025.8773827
ACACTAT59750.022.8917988
CACTATA68550.020.0581159
CACACTA23350.017.26694
AAACACT87450.016.2170036
CAAACAC90150.015.8910125
GAGCACA90700.015.556549
AGAGCAC94350.015.2599218
ACCAAAC99200.014.5154843
TAGCCCT54850.013.3886492
CCAAACA115500.012.7786534
GCCCTTA57750.012.5916484
GATCGGA119300.012.1983451
ACACACT39550.011.65055753
TACACAC40850.010.9272992
TATTTAG84350.08.96124659
GTAGCCC83550.08.884871
TCGGAAG166450.08.7806253
AAGAGCA179450.08.18372257
TACCAAA186100.08.0469292
Produced by FastQC (version 0.11.5)