FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849953
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA54100.025.2838847
ACACTAT59750.022.8930238
CACTATA70750.019.7407079
CACACTA22250.017.8040124
CAAACAC87350.016.566375
AAACACT89250.015.7294616
GCCCTTA57950.014.541784
ACCAAAC98400.014.2732543
TAGCCCT56250.013.44467452
CCAAACA111800.013.0779434
GATCGGA128650.011.5366641
AGAGCGT131300.010.9112828
ACACACT41200.010.6639013
GAGCGTC156600.09.654179
TATTTAG83350.09.5011149
GTAGCCC79750.09.3511161
TACACAC43250.09.3257862
CCCTTAT91050.09.2512445
AAGAGCG158000.09.1129797
TACCAAA168300.08.9442852
Produced by FastQC (version 0.11.5)