FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16085611
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGT121150.028.9530262
CCAGTCG179350.019.43421
AGTCGTA92800.018.4696793
AGTCGTG69800.017.2302443
TAGCAGG56150.09.4887947
TGCGTAG59050.08.6590213
GTCGTAT129100.08.3674944
CGTAGCA68000.07.94296655
AGTCGTC103900.07.0699283
GTTGCGT90250.06.7305891
GTCGTGT122750.06.39492374
TTGCGTA88750.06.24900442
GTAGCAG90800.06.10734946
GCGTAGC102150.05.14653444
Produced by FastQC (version 0.11.5)