FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17755120
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTTGATGGTGTAGATCT379330.21364541608279752Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA237200.023.7672861
TCGGAAG288300.019.8792923
AGAGCGT290000.019.8124148
ATCGGAA311450.018.8640232
GAGCGTC310850.018.2982149
AAGAGCG339800.017.1842277
GAAGAGC407100.014.6263836
CGGAAGA418550.013.933824
GGAAGAG427100.013.587455
TAGATCT109100.011.0342840-44
AGTGTAC113900.010.53134325-29
GTGTACT132750.09.15525425-29
GAGTGTA133900.09.06586925-29
GTAGATC137150.09.00853240-44
GTAGGGA140850.08.71054315-19
TAGGGAA148900.08.22995315-19
TGTAGGG147950.08.21466715-19
CTAGCGT13150.07.99391690-94
GTGTAGA160100.07.98700840-44
AGAGTGT158100.07.9696425-29
Produced by FastQC (version 0.11.5)