FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14080402
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG35200.018.1936788
TGAGTAG28300.014.4969193
AGTGAGT37450.012.3942851
GTGAGTA34650.011.8407012
TAGGAGG45850.09.8872237
GAGTAGG47850.09.6246864
CCTTTAG76050.09.1782754
TTAGCAT82250.08.22365957
CTTTAGC111700.06.8287755
TTTAGCA114500.06.4103956
CTAGGAG15400.06.231308550-54
AGTAGGA77150.06.15599355
AGCATGA136250.05.80937439
GGAGGGG94450.05.63771259
GTAGGAG90850.05.30688866
AGGAGGG100350.05.2345428
TCCTTTA164350.05.12393333
Produced by FastQC (version 0.11.5)