FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15183435
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT274280.18064423498371746TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA29450.038.3988343
CGACTAA24350.036.9755553
ATACTAA57700.023.5933533
TAGAGTA44200.023.1411782
TATACTA61450.022.9748972
CTATACT60350.022.9456671
GTTCCTA48450.018.5838472
GAGCACA259650.018.5504939
AGAGCAC262700.018.2530178
GTAGAGT55550.018.0110741
GATCGGA273750.017.7180771
CTCAGAT78650.016.961481
TCGGAAG297250.016.2593943
TACTAAC25950.015.2586914
AGAGTAA67400.015.0682523
GTCTAAA39050.014.7489414
TCGTCTA80350.014.7020312
TTCCTAA60150.014.6092483
ATCGGAA337500.014.5982812
CTACTAA44800.014.309213
Produced by FastQC (version 0.11.5)