FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16541203
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA156000.014.0291819
AGAGCAC172650.013.0098218
GATCGGA200850.011.4216131
TCGGAAG208450.010.813
ATCGGAA224600.010.39106752
AAGAGCA240900.09.3239757
AGTCACA51000.08.10267525-29
GGAAGAG304050.07.6715535
CAGTCAC65550.07.00704225-29
CGGAAGA355950.06.57321264
CGCTTAA67150.06.347110335-39
GCTTAAT69600.06.14437335-39
CCAGTCA75700.06.143605725-29
GAAGAGC397250.06.03481056
CACACGT80900.05.87326710-14
CTTAATC79350.05.75231535-39
CTCGTAT82850.05.422230740-44
TCACAAC78600.05.294165630-34
GTCACAA81650.05.237414425-29
TTAATCT85400.05.20991935-39
Produced by FastQC (version 0.11.5)