FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15112801
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT3527022.3337963624347333No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT1318900.8727038753438228TruSeq Adapter, Index 20 (97% over 38bp)
CGCGCATGGTGGATTCACAATCCACTGCCTTGATCCACTTGGCTACATCC301320.19938064426309857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCATG444650.0123.6429444
TATGCAT450550.0122.544763
GTTATGC452750.0122.114781
TTATGCA472550.0116.991182
TGAACGT505650.0108.4952859
TGCATGA510750.0107.806985
CATGAAC560800.098.108127
ATGAACG562600.097.743048
GCATGAA566550.097.201686
TCTAGAC406500.027.62147530-34
GACCTAG404950.027.6020135-39
CGCGCAT194650.027.4920851
ACCTAGC410950.027.3882635-39
CTAGACC406250.027.14575230-34
CTCTAGA413150.027.08277130-34
CCTAGCT409600.026.98626935-39
CCTCTAG413000.026.95662530-34
CTAGCTG410800.026.90973340-44
GTGGATT195250.026.7333189
GGTGGAT197350.026.485338
Produced by FastQC (version 0.11.5)