FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15112801
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT3283882.1729128835878937No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1340280.8868508226899832Illumina Single End PCR Primer 1 (100% over 50bp)
CGCGCATGGTGGATTCACAATCCACTGCCTTGATCCACTTGGCTACATCC275190.1820906660519119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCATG449900.0122.885444
GTTATGC457400.0122.0069661
TATGCAT456800.0121.26683
TTATGCA478150.0115.8488162
TGAACGT510550.0107.685519
TGCATGA516750.0106.862855
CATGAAC558300.098.7164847
GCATGAA564100.097.6632166
ATGAACG567050.097.193218
TCTAGAC412400.027.39959530-34
GACCTAG407350.027.32469635-39
ACCTAGC411450.027.25883935-39
CTCTAGA412700.026.94015130-34
CTAGACC410000.026.92795630-34
CCTAGCT409500.026.81914735-39
CCTCTAG411150.026.72658230-34
CTAGCTG410050.026.65045240-44
GTCTTAG406550.025.71880580-84
TCTTAGT404200.025.70447780-84
CTTAGTG393900.025.54686480-84
Produced by FastQC (version 0.11.5)