FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14258439
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCT40700.023.98470520-24
CTAGCTA41600.023.60424820-24
GCTAACT49550.019.9940125-29
CTAACTA49600.019.82869725-29
ACCTAGC14650.019.0661620-24
AGCTAAC52150.018.93951820-24
GGTCCTA52900.018.56202535-39
GTCCTAA57750.017.15272535-39
CCCTAGC15350.017.07112520-24
TCCTAAG58700.016.850635-39
TAGCTAA61600.016.40799520-24
CCTAAGG61650.016.06758140-44
TCCTAGC16900.015.5054320-24
CTAAGGT66550.015.07925540-44
TAGCGAA81050.012.61244545-49
GCCTAGC16550.012.52747720-24
GTAGCGA83150.012.34585745-49
TAAGGTA86700.011.89019940-44
CCAGTGA89100.011.21508450-54
TAACTAT95650.010.73395425-29
Produced by FastQC (version 0.11.5)