FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16551991
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT1304340.7880260447217498No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT1023910.6186023179930439TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGC231350.088.070761
ATGCATG235100.086.5396964
TATGCAT251550.081.108993
TGAACGT257200.078.599429
TTATGCA283450.072.030132
TGCATGA299950.067.901595
CATGAAC323400.062.9779857
ATGAACG324250.062.879518
GCATGAA344450.059.3173946
AGAGCAC488750.033.4523168
GAGCACA494100.033.1044779
GATCGGA566650.029.2280271
TCGGAAG568800.029.1342433
AAGAGCA574600.028.5795577
GAAGAGC595600.027.7411086
TCTAGAC152850.027.00732230-34
ACCTAGC153550.026.87544335-39
GACCTAG153200.026.84285435-39
CTAGACC152700.026.6378230-34
CCTAGCT152700.026.48755835-39
Produced by FastQC (version 0.11.5)