FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14809940
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA50800.019.2730859
GCCACTC66450.014.8423148
AGCCACT89200.011.4603947
AAGCCAC100050.010.2895166
GGCCTAA20000.010.00699675-79
CCGTTAA65850.07.2154394
TACAAAG155250.07.1941512
ATACAAA178300.07.0010211
CGTTAAA69850.06.69920065
ACAAAGC164500.06.5693533
CTCACTA34650.06.39911215-19
ACTCACT36350.06.258278415-19
TCCGTTA85550.05.89488463
GTTAAAT81600.05.6463256
AAAGCCA205200.05.5431395
CCCCTAA41500.05.4472604110-114
ACACACT44700.05.186235450-54
TACACAC46450.05.05284350-54
Produced by FastQC (version 0.11.5)