FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16123052
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG25650.027.5068285
CTTAGAG33000.021.1621447
CCTTAGA33400.020.4775946
TTAGAGC36500.020.1191278
CCCCTTA35000.019.7471774
CTCATAT89350.016.1152574
CATATCA112850.013.0783846
ATCAATC110250.012.2115729
TAGAGCC65600.010.8652549
GCTCATA116900.010.4083223
TATCAAT135350.010.0000328
TCATATC149600.09.6731145
TAACTCG31150.09.66085825-29
CTAACTC38900.08.84658825-29
AACTAAC39900.08.69704325-29
ATATCAA171350.08.6553567
ACTAACT45700.07.750797725-29
AAGCTCA198750.07.47926861
AGCTCAT189350.07.4532862
ACTAATC44800.07.102915350-54
Produced by FastQC (version 0.11.5)