FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15736493
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC474220.3013504978523487No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTAT199550.12680716090935892TruSeq Adapter, Index 1 (97% over 37bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG174010.11057736943040614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA141700.026.2654239
AGAGCAC142000.025.9074868
GATCGGA146200.025.3331341
ACCACTA77850.021.7321118
ACGTTAA49800.018.0707474
GTTACGT60800.016.4281851
TACGTTA58200.016.3288673
TCGGAAG240300.015.5795413
CACCACT118800.014.9683257
AAGAGCA260100.014.6145767
CCTAGAC23050.014.553839120-124
GAAGAGC264050.014.31415756
CCACTAC121900.014.0551979
ATCGGAA274800.013.8577592
ACAACAC124250.013.8486233
ACACCAC131650.013.1245096
CTAGACC25500.013.099067120-124
GGAAGAG329550.011.7749785
CGTTAAA80250.011.6624825
ATACAAC159650.011.6451171
Produced by FastQC (version 0.11.5)