FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14529826
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG19002.7284841E-118.7134378
GAGCACA124150.07.07340249
AGAGCAC130200.06.52358448
GGGGGAG74850.06.44301757
GATCGGA138950.06.276951
AAGGGGG82300.06.12555174
CTATTAG57250.06.0170361
CCTCTAA46850.05.94475841
CTATCGT107850.05.77642351
CTATAGA62950.05.5886351
GTATCGA123600.05.45543771
CTAGCCA19550.00256393455.2485761
TAGATCG40950.05.2389436140-144
CTAAGGG19900.0032009615.15626431
GTGTGAG41601.0801159E-85.10934161
TATCGAG107300.05.10380652
CTTGTGA100650.05.097351
CTATCGA82400.05.06999871
CTATATC168050.05.0591751
Produced by FastQC (version 0.11.5)