FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13521018
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAG30053.274181E-116.70881843
GTCCCTA19702.0406787E-66.578683
AGTGAGT29305.7480065E-96.14334441
GTGAGTA34706.903065E-95.6023062
GCTATAC69100.05.3140389
GAGTAGG40651.715307E-95.31365354
TACACTA28102.0592724E-55.1245552
TCCCTAA24103.0322804E-45.07883834
TGCTATA59950.05.04420388
Produced by FastQC (version 0.11.5)