FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18815688
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGACGGAGTGTAGATCT596350.31694296801690164Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA301100.033.3118321
AGAGCGT304000.032.8300558
TCGGAAG348950.029.0320853
GAGCGTC354900.028.4256639
AAGAGCG364200.027.7988827
ATCGGAA379650.026.6081332
GAAGAGC424200.024.0027316
GGAAGAG456800.022.3350265
CGGAAGA483900.021.0547684
TTAGACG116600.016.78254530-34
GTGTTAG115900.016.77209325-29
GTTAGAC117300.016.75604630-34
TGTTAGA129350.014.97244630-34
TAGATCT141750.014.48598140-44
AGTGTTA143900.013.67869425-29
GAGTGTA147250.013.43595635-39
AGTGTAG148050.013.22762740-44
GGAGTGT164800.012.23229635-39
GAGTGTT163450.012.21879725-29
GTAGATC177400.011.77782940-44
Produced by FastQC (version 0.11.5)