FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15845808
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCCC267240.16865028277510366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA85950.044.8119358
ACACTAT94800.041.3879557
AACACTA99650.039.3013426
CAAACAC108500.035.564844
AAACACT130050.030.0036935
ACTATAG131350.029.706749
CCAAACA156350.025.1431063
TCCAAAC191750.020.5507832
ATCCAAA289500.013.9092761
AGGCCCC63700.012.74849745-49
GGCCCCA65800.012.4291645-49
CCCCTCA65350.012.38307485-89
GGCCCCT71450.011.48709985-89
TAGCCCT64150.011.34232
TACACTC75950.010.787208115-119
GAGGCCC81000.010.487926540-44
TGGCCCC81000.010.20386285-89
GCTCTCA80400.010.19029615-19
AGTACAC84900.010.073975110-114
TGAGGCC84400.09.84364640-44
Produced by FastQC (version 0.11.5)