FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15845808
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCCC258420.1630841418752518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA87300.047.912288
AACACTA98450.041.9740756
ACACTAT99200.041.439017
CAAACAC107150.038.5655174
ACTATAG130200.032.2913979
AAACACT129250.031.9160425
CCAAACA158400.026.1786233
TCCAAAC189050.022.1627862
ATCCAAA282600.015.0152911
AGGCCCC63500.013.35530445-49
CCCCTCA64600.013.2397185-89
TAGCCCT69450.013.0609982
GGCCCCA68100.012.70689945-49
GGCCCCT70200.012.40917285-89
TACACTC78100.011.11671115-119
TGGCCCC80250.010.96278185-89
GAGGCCC80050.010.88187640-44
GCTCTCA81550.010.84061415-19
GTAGCCC83100.010.7408021
TGAGGCC85100.010.21920540-44
Produced by FastQC (version 0.11.5)