FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005185402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005185402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14926514
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG55100.026.5230546
TAGTCAT70400.024.8492156
GTAGCAT61500.023.8797519
CTGTAGC62100.023.4172537
TATAGTC83400.021.1486194
TGTAGCA71000.020.8873628
TATATAG92700.019.5779512
ATAGTCA90650.019.2188175
GAGCACA114500.017.6046439
ATATAGT111250.016.4429653
AGTCATT82750.015.8335957
AGAGCAC130900.015.3440238
TAGTTAG23700.012.94031130-34
GCCTGTA115850.012.9254715
GATCGGA162950.012.7155191
ACGTTAG25300.012.52014170-74
GATCTAA24050.012.5133245-49
CGTTAGT26100.012.0260570-74
TCGGAAG190050.011.0652543
ATATATA165900.010.9994481
Produced by FastQC (version 0.11.5)